Spectrophotometric Estimation of Chlorzoxazone and Diclofenac Sodium in Synthetic Mixture by First Order Derivative Spectrophotometry

Abstract

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical first order derivative spectrophotometry method for the simultaneous determination of Chlorzoxazone and Diclofenac Sodium in synthetic mixture. The absorbance values at 276 nm and 288 nm of first derivative spectrum was used for the estimation of Chlorzoxazone and Diclofenac Sodium, respectively without mutual interference. This method obeyed beer’s law in the concentration range of 2-24 μg/ml for both Chlorzoxazone and Diclofenac Sodium, respectively. The method was successfully applied to laboratory prepared mixture because no interference from the mixture excipients was found. The suitability of this method for the quantitative determination of Chlorzoxazone and Diclofenac Sodium was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of Chlorzoxazone and Diclofenac Sodium in mixture. The results of analysis have been validated statistically and by recovery studies.