Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of PARACETAMOL and PAMABROM in Bulk and Pharmaceutical Dosage Form

Abstract

Two methods for simultaneous estimation of Paracetamol and Pamabrom in combined tablet dosage form have been developed using Dist. water as a solvent. The first UV spectrophotometric method was a determination using the simultaneous equation method at 245.0 nm and 279.0 nm. The second UV spectrophotometric method is the Q – analysis (absorption ratio) method, which involves the formation of absorbance equation at 265.0 nm (iso absorptive point) and at 279.0 nm the maximum absorption of Pamabrom. The linearity ranges for Paracetamol and Pamabrom were 4-14 μg/ml and 2-12 μg/ml respectively. The accuracy of the methods was assessed by recovery studies was found to be 98.06%and 98.95 for simultaneous equation method and 98.65% and 99.54% for Q analysis (absorption ratio) method for Paracetamol and Pamabrom respectively. These methods are simple, accurate and rapid; those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories.