“Developmentandvalidation of dualwavelength method for Simultaneous Estimation of Ebastine and Phenylephrine hydrochloride in Bulk and Pharmaceutical Dosage Form

Abstract

A simple, rapid, accurate and precise Dualwavelength spectrophotometric method was developed for the simultaneous determination of Ebastine and Phenylephrine hydrochloride inpharmaceutical dosage form. The Dual wavelength spectrophotometric method was based on the determination of difference in absorbance of both the drugs at the respective Isoabsorptive point (IAP) of other drug. The Dualwavelength spectra were obtained using methanol as a solvent and the determinations were made at 209.2 nm& 221.6nm (IAP of Phenylephrine) for Ebastine and 253.0 nm& 216.0nm (IAP of Ebastine) for Phenylephrine hydrochloride. The linearity was obtained in the concentration range of 4-24μg/ml for both drugsand correlation coefficient (r2) were found to be 0.9998 and 0.9992 for Ebastine and Phenylephrine hydrochloride respectively. The percentage purity of drugs in combined tablet dosage form was found to be 99.33 % for Ebastine and 98.75 % for Phenylephrine hydrochloride.The % recoveries were found to be 99.84% for Ebastine and 99.18% for Phenylephrine hydrochloride. The method was found to be simple, accurate and precise and was applicable for the simultaneous determination of Ebastine and Phenylephrine in tablet dosage form.