Preliminary Phytochemical Screening and In Vitro Thrombolytic Potential of The Methanolic Extract of Enhydra Fluctuans Lour (Leaves)

Abstract

Investigation with methanol extract of Enhydra fluctuans Leaves was carried out to evaluate its thrombolytic potential andto find out possible phytochemical nature.Preliminary phytochemical analysis of methanolic extract showed the presence of alkaloids, carbohydrates, saponins, phenols, tannin, proteins and amino acids, gum and mucilages and triterpenes.A quick & rapid methodology (In-vitro Thrombolytic model) was applied to find out their thrombolytic potential where streptokinase and distilled water were employed as positive and negative controls, respectivelyThe thrombolytic nature of the plant was found significant (p < 0.001) when compared with the negative control (water) at different dosesThe plant showed significant clot lysis, i.e. 15.98 ± 2.88%, 21.56 ± 3.32%, 24.78 ± 1.59%, 28.67± 4.51%, and 31.25± 1.96% at 2, 4, 6, 8, and 10 mg/ml concentrations respectively, while the standard (streptokinase) and negative control (distilled water) showed 40.13 ± 1.65% and2.56±1.23% clot lysis respectively.Through our study, it was found that E. fluctuanspossess thrombolytic property that could lyse blood clots in vitro; however, in vivo clot dissolving properties and active component(s) of this extract for clot lysis are yet to be discovered.