Reverse Phase HPLC Method for Estimation of Acelofenac and Pregabaline in Combined Dossage Form

Abstract

A simple, precise, accurate and rapid reverse phase high performance liquid chromatographic isocratic method has been developed for the estimation of Aceclofenac (ACF), and Pregabaline in tablet dosage form. The mixed standard solution containing of Pregabalin and Aceclofenac were chromatographed with mobile phase of different ratio of Buffer and acetonitrile, respectively at different pH values. (0.02MKH2PO4)-ACN-TEA 65-35-0.1 ph4.5 with H3PO4) , RP-18 (E-Merck, 5 μm, 4.0 x 250 mm) column was selected over the other columns. This RP-18 (E-Merck, 5 μm, 4.0 x 250 mm) column has an embedded polar groups and which are more stable at lower pH and high Carbon loads, which provide high peak purity and more retention to polar drugs and facilitates the separation of impurity peaks within a very short run time.  Injection volume – 10 μl  Column temperature – 250 C  Sample temperature – 250 C  Flow rate- 1.5ml/min  λmax- 205nm